Enzymes and Inhibitors
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
Thermo Scientific™ SalI (10 U/μL)
The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.
Thermo Scientific™ Tango Buffer (10X)
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ XhoI (10 U/μL)
Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Thermo Scientific™ AloI (2 U/μL)
Cut at ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites with AloI restriction enzyme, which performs best at 30°C in R buffer.
Thermo Scientific™ BplI (5 U/μL)
Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer.
Invitrogen™ Nitro blue Tetrazolium Chloride (NBT)
Use to assess cell viability as function of redox potential
Thermo Scientific™ FastDigest TaqI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
Thermo Scientific™ FastDigest SmaI
The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (isoschizomers: TspMI, XmaCI; neoschizomer: XmaI).
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Thermo Scientific™ SspI (10 U/μL)
The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.